quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. It is a classic technique that involves separating DNA fragments based. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. Faça loginto View Organizational & Contract. For. BioTechniques. quantification of low amounts of dsDNA. RESULTS Dramatic differences with. Southern blot analysis reveals information about DNA identity, size, and abundance. Anal Slot Blot Hybridization. J Forensic Sci. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. . quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. GAPDH was used as a sample loading control. ; 30 Quantification Kit. Dot blotting is an ideal technique for quickly. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups.